Mechanism of Propionate Formation by Selenomonas ruminantium, a Rumen Micro-organism
نویسندگان
چکیده
منابع مشابه
Multiple lactate dehydrogenase activities of the rumen bacterium Selenomonas ruminantium.
The lactate utilizing strain of Selenomonas ruminantium 5934e was found to contain three lactate dehydrogenase (LDH) activities in sonicated cell extracts. One activity, an NAD dependent L-LDH (L-nLDH) was measured at 15-fold greater levels in extracts of cells grown to mid-exponential phase on glucose compared to cells grown to the equivalent growth stage on DL-lactate. A second nLDH activity ...
متن کاملAmmonia assimilation and glutamate formation in the anaerobe Selenomonas ruminantium.
Selenomonas ruminantium was found to possess two pathways for NH4+ assimilation that resulted in net glutamate synthesis. One pathway fixed NH4+ through the action of an NADPH-linked glutamate dehydrogenase (GDH). Maximal GDH activity required KCl (about 0.48 M), but a variety of monovalent salts could replace KCl. Complete substrate saturation of the enzyme by NH4+ did not occur, and apparent ...
متن کاملCharacterization of lateral flagella of Selenomonas ruminantium.
Selenomonas ruminantium produces a tuft of flagella near the midpoint of the cell body and swims by rotating the cell body along the cell's long axis. The flagellum is composed of a single kind of flagellin, which is heavily glycosylated. The hook length of S. ruminantium is almost double that of Salmonella.
متن کاملFactors affecting lactate and malate utilization by Selenomonas ruminantium.
Lactate utilization by Selenomonas ruminantium is stimulated in the presence of malate. Because little information is available describing lactate-plus-malate utilization by this organism, the objective of this study was to evaluate factors affecting utilization of these two organic acids by two strains of S. ruminantium. When S. ruminantium HD4 and H18 were grown in batch culture on DL-lactate...
متن کاملEffects of laidlomycin propionate and monensin on glucose utilization and nutrient transport by Streptococcus bovis and Selenomonas ruminantium.
The objective of this study was to compare the effects of laidlomycin propionate and monensin on cell growth, glucose fermentation, and glucose uptake in Streptococcus bovis strain JB1 and Selenomonas ruminantium strain HD4. Experiments were also conducted to compare the effects of both ionophores on sodium-dependent serine transport and cell yield in S. bovis. Batch cultures (500 mL) of each b...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1970
ISSN: 0022-1287
DOI: 10.1099/00221287-61-1-1